If the acidic collagen solution has a protein concentration of 4 mg/mL (in 0.02 N-acetic acid), the components necessary for preparation of 75 mL neutralization buffer are the following:?10 PBS (Corning 46-013-CM), 10 mL; HEPES (1 N, ThermoFisher 15630080, pH?= 7.4), 2 mL; sodium bicarbonate (7.5% w/v, Corning 25-035-CI, pH?= 8), 6 mL; sodium hydroxide … Continue reading If the acidic collagen solution has a protein concentration of 4 mg/mL (in 0